微量元素铬载体酵母摇瓶发酵研究

STUDIES ON FLASK FERMENTATION OF TRACE ELEMENT CR-RICH YEAST

实验选择了啤酒酵母 2 0 16菌株为铬载体酵母生产菌株 ,通过摇瓶发酵实验 ,对三氯化铬的添加工艺进行了研究探索。确定了铬酵母发酵培养的适宜加铬条件 :在发酵开始的8h内分批流加三氯化铬 ,总加入量为 5× 10 4mol/L ,经过 12h摇瓶发酵可得铬酵母的生物量为 1 5 5 g/ 10 0mL ,酵母含铬量为 1,10 0 μg/ g以上 ,酵母细胞对铬离子利用效率是 6 0

From 6 stains of yeasts, Saccharomyces cerevisiae 2016 which was suitable for Cr rich yeasts fermentation was selected Concentration and additive method of CrCl 3 were investigated by liquid culture and the optimum conditions of Cr rich yeasts were obtained The optimum concentration of CrCl 3 were 5×10 4 mol/L that were added to liquid medium at the initial 8 h of culture time At this condition, the biomass of Cr rich yeasts was 1 55 g dry weight per 100 milliliter, Cr content in yeast cells was above 1100 μg/g, the utilizable rate of Cr ion was about 60%