染矽尘小鼠肺组织中转化生长因子β_1表达的免疫组织化学检测

Immunohistochemical method for detecting the expression of transforming growth factor-β_1 in lung tissues of silica-treated mice

目的 观察染矽尘小鼠肺组织转化生长因子 β1(TGF β1)蛋白水平的改变 ,研究其在矽肺发病中的意义。方法 采用暴露式气管内注入法给小鼠一次性染矽尘 (0 .2g/kg)。染尘后第 1、3、5、7、14、2 8天每组各处死 8只小鼠 ,取肺组织 ,用免疫组织化学技术检测TGF β1在小鼠肺组织蛋白水平的表达。结果 对照组小鼠肺组织中免疫组化染色呈弱阳性 (± ) ,可见少量TGF β1蛋白 ;染尘组小鼠肺TGF β1表达明显增强 ,免疫组化染色结果为 +～ +++,染尘后第 7～ 14天达高峰。染尘组小鼠肺出现TGF β1表达阳性肺泡巨噬细胞 ,第 5天达高峰 ,其阳性肺泡巨噬细胞百分率为 93.4 %± 2 .8% ,与对照组 (42 .2 %± 12 .0 % )相比 ,差异有显著性 (P <0 .0 1)。结论 TGF β1在矽肺早期的发生、发展过程中可能有重要作用。

Objective To investigate the protein expression of transforming growth factor-β_1(TGF-β_1) in lung tissues of silica-treated mice. Methods The experimental mice were divided into control and silica groups.0.2 g/kg body weight of silica was injected intratracheally in mice of silica group.Samples of lung tissue were collected 1,3,5,7,14 and 28 d after injection.The immunohistochemical method was used to analyze the protein expression of TGF-β_1. Results In control mice,the expression of TGF-β_1 in lung tissue was slightly positive while it was markedly increased in silica-treated mice.The expression was significantly elevated from the 7th day to 14th day.The expression in alveolar macrophages reached the peak on the 5th day[(93.4%±2.8%) vs (42.2% ± 12.0%) , P < 0.01] . Conclusion TGF-β_1 may play an important role in early development of silicosis.