摘要
本文主要对假密环菌(亮菌)的液体深层发酵工艺条件进行了试验研究,以菌丝体干重为指标确定了碳、氮源的最佳组成,并研究了发酵条件对菌丝体得率的影响,确定了以玉米粉5%,豆粕1%,酵母膏1%,KH2PO40.1%,MgSO40.05%为主的发酵培养基。培养条件为:接种量5%,温度25-28℃,转速130r/min,培养时间11～13d。
In this paper,the liquid fermentation conditions of Liangjun are studied.By means of mycelical dry weight,optimal fermentation medium containing carbon source was determind.The effect of different culture conditions on the mycelical dry weight are also studied.The optimum liquid fermentation medium is listed as follows:5% maize powder,1% soy bean cake,1% yeast extract,0.1% potassium dihydrogen phosphate,0.05% magnesium sulfate.The culturing conditions of inoculation volume,temperature,rotation rate and culture period are 5% liquid seeds,25～28℃,130 r/min and 11～13 days respectively.
