维拉帕米对离体家兔阴茎海绵体平滑肌舒张作用的实验研究

Experimental study of verapamil on the relaxation of isolated rabbit corpus cavernosum tissues

目的 初步探讨维拉帕米 (VP)对离体兔阴茎海绵体平滑肌的舒张作用及其机制。方法 离体家兔阴茎海绵体平滑肌条实验方法 ,观察VP对阴茎海绵体平滑肌的舒张效应。结果 VP可舒张去氧肾上腺素 (PE)诱导的阴茎海绵体平滑肌收缩作用 ,最大舒张效应为 (4 6 .3± 2 .1) % (P <0 .0 1) ,且呈浓度依赖性。 10 -6mol·L-1,10 -5mol·L-1和 10 -4mol·L-1VP均可使PE引起的浓度 -效应曲线右移 ,最大收缩反应降低 ,10 -4mol·L-1VP拮抗作用更显著。结论 VP有明显的舒张阴茎海绵体平滑肌作用 ,并呈浓度依赖性。

Objective To study the relaxant effect and mechanism of verapamil (VP) on the isolated rabbit corpus cavernosum tissues in vitro.Methods The relaxant effect of VP on corpus cavernosum was observed by using experimental method of cavernosal smooth muscle strips.Dose responses to phenylephine (PE) were recorded.Results VP concentration dependently depressed the response to PE and was effective in inhibiting adernergically induced contractions.The maximum percent relaxation of cavernosal strips was (46.3±2.1)% (compared with control;(17.5±4.8%, P <0.01).Conclusions VP was significantly effective in relaxing cavernosal smooth muscle induced by PE in vitro.