丝氨酸羟甲基转移酶基因(glyA)的克隆

Cloning of the glyA gene encoding the serine hydroxymethyltransferase

采用PCR方法从大肠杆菌K12染色体DNA中克隆出1.5kb的DNA片段,并将其与pUCm-T载体连接转入大肠杆菌JM109中。DNA测序分析证明该片段含有一个完整的丝氨酸羟甲基转移酶基因(glyA)。

A 15 kb DNA fragment was amplified from the Escherichia coli strain K12 by PCR technique Then, this DNA fragment was ligated with pUCm-T vector and transformed into the Ecoli strain JM109. DNA sequencing showed this DNA fragment contained a perfect serine hydroxymethyltransferase gene (glyA)