乙肝preS_1抗原与HBV-DNA拷贝数的对比研究

Comparison Research of HBV preS_1 Antigen and HBV-DNA Copies

目的进一步探讨乙肝preS1抗原与HBV-DNA的关系及临床意义.方法乙肝preS1抗原采用双抗体夹心ELISA法检测,HBV-DNA采用荧光定量PCR法检测.结果 110例急、慢性乙型肝炎及携带者preS1抗原阳性63例,HBV-DNA≥103(拷贝数/m1)有62例.其中DNA≥103中,preS1抗原阳性55例(P＜0.01);59例HBeAg阳性中48例preS1抗原阳性(P＜0.01).结论乙肝preS1抗原与HBV-DNA的复制密切相关,它可作为乙肝早期诊断、疗效评估等的新指标.

Objective To further discuss relationship and clinical value between HBV preS1 antigen and HBV-DNA copies. Methods HBV preSi antigen was detected by sandwich ELISA. HBV-DNA was detected by fluorescent quantification PCR. Results Among 110 patients with acute,chronic hepatitis B and HBsAg carriers,there were 63 cases with preS1 positive and 62 cases with HBV-DNA≥lO3 copies/ml. There were 55 cases with preS1 positive in 62 cases with HBV-DNA≥103(P<0. 01). There were 48 cases with preSi positive in 59 cases with HBeAg positive (P<0. 01). Conclusion There was closely correlation between HBV preS1 and HBV-DNA replication. HBV preS1 antigen can be used as new marker of early diagnosis and evaluating the efficacy of therapy in hepatitis B.