幽门螺杆菌(SS1)感染及其胃炎的小鼠模型

THE MOUSE MODEL INFECTED WITH HELICOBACTER PYLORI

目的 :建立感染幽门螺杆菌 (Helicobacterpylori,H pylori)SS1株BALB/c小鼠感染模型 ,研究H pylori胃内定植及胃黏膜病理变化。 方法 :BALB/c小鼠胃内分别接种体外培养的H pyloriSS1株 (实验组 )或PBS(对照组 ) ,组织学方法评价H pylori定植及胃黏膜病理变化。结果 :所有对照组小鼠胃组织未见H pylori定植 ,胃组织也未见明显的炎症反应 ;而所有实验组小鼠在感染H pylori 12周后 ,胃黏膜表面的黏液层及胃小凹顶端可见大量H pylori,胃体及胃窦交界处、胃体及胃底交界处最多 ;胃组织可见到不同程度的炎性反应 ,感染H pylori 2 4周后 ,胃组织炎性反应加重。结论 :用胃内接种方法建立了小鼠H pylori感染及其相关性胃炎的模型。

Objective:BALB/c mouse were infected with Helicobacter pylori ( H.pylori ) SS1 strain,the H.pylori colonization and the pathogeny of stomach were evaluated.Methods: H.pylori (SS1),grown under microaerobic conditions in vitro ,or PBS were orally inoculated in experimental group or in control group separately.Stomach tissues were evaluated for the inflammation and the H.pylori colonization. Results: All of the mouse in control group,there was no H.pylori and no significant character of inflammation 2 weeks postchallenge;for all of the mouse in experimental group,a large number of H.pylori in the mucus overlaying the surface and in the top of the gastric pits with highest density in the transition zones between the antrum and body and between the body and the cardic equivalent,gastritis to varying degree could be observed.24 weeks after challenge,the gastritis become severy.Conclusion:BALB/c mouse were infected with H.pylori (SS1) strain by the method of oral inoculation and H.pylori associted gastritis was developed.