胶体金法与TPPA法检测梅毒特异性抗体的对比研究

THE ASSESSMENT OF TPAB FOR DETECTION OF TREPONEMA PALLIDUM BY COMPARISION WITH TPPA

目的 :探讨梅毒螺旋体特异性抗体胶体金法 (TPAb)在临床工作中的应用。方法 :以梅毒累旋体明胶凝集试验 (TPPA)为“金标准” ,同时用TPAb法检测梅毒高危人群的血清。结果 :两种方法经 χ2 检验P >0 0 5 ,差异没有显著性 ,Kappa值为 0 86 ,TPAb法其敏感性 88 9% ,特异性 97 8% ,准确性 93%。结论 :TPAb法可推荐用于血清学梅毒特异性抗体检查 。

Objective:To assess the application of TPAb immunogold.labeling technique for the detection of.Treponema Pallidum in clinic.Methods:To use TPAb and TPPA,which is taken as a 'gold criteria',to detect Treponema Pallidum in the sera of the people at high risk of syphilis respectively and simultaneously so as to make the comparison. Results: The two detection methods showed no apparent discrepqncies as a result of χ 2 test with P >0.05 and Kappa value=0.86.The sensitivity,the specificity and the accuracy resulting from TPAb was 88.9%,97.8% and 93% respectively.Conclusion:The application of TPAb for the detection of the specific antibodies to Treponema Pallidum in human sera is advisable and conducive to a rapid diagnosis of syphilis in clinic.