大豆异黄酮对衰老小鼠羟脯氨酸等物质变化的影响

Effect of Soybean Isoflavones on the Contents of Hydroxyproline and Peroxide Lipid and on the Activities of Glutathione Peroxidase in Aged Mice

以 13月龄昆明种NIH雌性小白鼠为材料 ,通过小鼠自由饮用含不同剂量大豆异黄酮的溶液 ,就大豆异黄酮对老龄小鼠脂质过氧化物含量、谷光甘肽过氧化物酶活性及皮肤羟脯氨酸含量变化的影响作了初步探讨。结果显示大豆异黄酮可显著提高衰老小鼠皮肤羟脯氨酸含量 ;较高剂量的异黄酮可显著降低老龄小鼠全血脂质过氧化物含量 ,且随着老龄小鼠月龄增大 ,大豆异黄酮降低脂质过氧化物作用显著 ;大豆异黄酮对衰老小鼠全血谷胱甘肽过氧化物酶活性的提高作用不显著 ,但在高剂量下 ,对小鼠在衰老过程中全血谷胱甘肽过氧化物酶活力下降有一定的延缓作用。提示 ,大豆异黄酮具有一定的延缓机体衰老的功能。

The contents of hydroxyperoline and peroxide lipid in skin and blood of 14 month-aged mice,which had been fed on soybean isoflavones solution for more than 30 days,were determined respectively,and the activity of glutathione peroxidase in blood was analyzed.The results showed that,after 45 days,the hydroxyperoline contents in mice skin of high and middle dosage groups(fed on 200mg/100ml and 40mg/100ml soybean isoflavones solution respectively)increased( t<t _ 0.05 ) comparing with control group, while the peroxide lipid contents in mice blood of high dosage group decreased (t_ 0.05 ). High dosage of soybean isoflavones is helpful to maintain the activity of glutathion peroxidase in aged mice.