五种螯合剂对镉致小鼠脏器脂质过氧化作用的影响

Comparative Effects of Chelating Agents on Lipid Peroxidation in Mice Organs by Acute Exposure to Cadmium

[目的 ]开发高效低毒的新型解毒剂。 [方法 ]研究镉染毒组小鼠和N 对羟甲苯甲基 D 葡糖二硫代氨基甲酸钠 (HBGD)、N 苯甲基 D 葡糖二硫代氨基甲酸钠 (BGD)、二乙基二硫代氨基甲酸钠 (DDTC)、二巯基丙醇 (BAL)、乙二胺四乙酸 (EDTA)等五种螯合剂治疗组小鼠各脏器的脂质过氧化物 (LPO)值及血清乳酸脱氢酶 (LDH)、天冬氨酸转氨酶(AST)和丙氨酸转氨酶 (ALT)活性的变化。 [结果 ]氯化镉染毒 ( 2 5mgCd/kg ,腹腔注射 )后小鼠多数脏器LPO值和血清LDH、AST及ALT活性显著高于对照组。染镉 3 0min后用各螯合剂治疗 ( 4 0 0 μmol/kg ,腹腔注射 ) ,2 4h后除EDTA外 ,其余螯合剂均对镉引起的各脏器LPO值及血清LDH、AST、ALT活性的升高有显著的抑制作用 (P <0 0 5 ) ;染镉 2 4h后 ,注射各螯合剂 ,治疗 2 4h后 ,HBGD、BGD和DDTC显著抑制了上述各指标的升高 (P <0 0 5 )。 [结论 ]HBGD和BGD对镉致小鼠各脏器LPO值和血清LDH、AST及ALT活性升高的抑制效果最好 。

Objective] To develop new antidotes of cadmium(Cd). [Methods] N p hydroxymethyl benzyl D glucamine dithiocarbamate(HBGD),N benzyl D glucamine dithiocarbamate(BGD),diethyldithiocarbamate(DDTC),2,3 dimercaptopropanol(BAL),and ethylene diaminetetraacetic acid(EDTA) were studied for their effects on lipid peroxidation(LPO) in most organs as well as activity of lactate dehydrogenase(LDH),aspartate transcarbamoylase(AST) and alanine aminotranferase(ALT) in serum of mice induced by acute exposure to cadmium. [Results] Mice were injected intraperitoneally with CdCl 2(2 5 mgCd/kg) and 30 min or 24 hr later,they were injected intraperitoneally with chelating agents (400 μmol/kg). Cd injection increased LPO in most organs and the activity of LDH,AST and ALT in serum of mice. At 30 min after Cd treatment,the chelating agents other than EDTA significantly depressed increase in LPO and in activity of LDH,AST and ALT( P <0 05). At 24h after Cd treatment,HBGD,BGD and DDTC effectively prevented increase in LPO and in activity of LDH,AST and ALT( P <0 05). [Conclusion] Treatment with HBGD or BGD is more effective than that with other chelating agents in preventing the changes caused by acute exposure to Cd,so HBGD and BGD are more effective antidotes of Cd.