茶多酚对糖尿病大鼠的保护作用

The Experimental Study of Tea Polyphenols(TP) on Diabetic Rats

[目的 ]探讨茶多酚对糖尿病并发症的保护作用及其机制。 [方法 ]链脲佐菌素 (STZ)诱发的糖尿病大鼠用茶多酚干预后 ,检测血清尿素氮 (BUN) ,肌酐 (CR) ,甘油三酯 (TG) ,胆固醇 (TC)及过氧化氢酶 (CAT) ,同时做肾脏病理检测。 [结果 ]与阴性对照组比较 ,糖尿病组大鼠血清尿素氮、肌酐、甘油三酯、胆固醇分别为 ( 2 1 85± 1 85 )mmol/L ,( 3 16±11) μmol/L ,( 3 3 4± 0 4)mmol/L和 ( 2 0 2± 0 3 2 )mmol/L ,明显高于对照组的 ( 5 47± 0 46)mmol/L ,( 78± 2 ) μmmol/L ,( 1 13±0 13 )mmol/L和 ( 1 5 4± 0 0 1)mmol/L。过氧化氢酶活性 ( 12 9 18± 7 6)U低于对照组的 ( 2 2 6 61± 11 4)U ,差异具有显著性 ;糖尿病 +茶多酚组的血清尿素氮、肌酐、甘油三酯及胆固醇含量分别为 ( 13 2± 0 2 )mmol/L ,( 2 17 3± 13 5 )mmol/L ,( 1 82± 0 63 )mmol/L和 ( 1 17± 0 0 8)mmol/L ,较糖尿病组明显降低 ,而过氧化氢酶活性为 ( 175± 1 65 )U ,较糖尿病明显升高。肾脏病理学显示 :糖尿病所致肾损伤主要表现为肾小球肥大 ,肾小球基底膜增厚 ,并伴有间质轻度炎症反应 ,糖尿病+茶多酚组肾小球结构较糖尿病组完整且清楚 ,肾小球轻度肥大。 [结论

Objective] To study the protective effects and mechanism of TP on diabetic nephropathy. [Methods] TP was given to streptozocin induced diabetic rats as treatment. After treatment the level of urea nitrogen(BUN),creatinine(Cr),triglycerinate(TG),total cholesterol(TC) and catalase hydrogen peroxide(CAT) were measured,while tissue of kidney was observed by microscope. [Results] The content of BUN,Cr,TG and TC in diabetic rats was (21 85±1 85)mmol/L,(316±11)μmol/L,(3 34±0 4)mmol/L and (2 02±0 32)mmol/L,respectively much higher than the corresponding value (5 47±0 46)mmol/L,(78±2)μmmol/L,(1 13±0 13)mmol/L and (1 54±0 01)mmol/L in control group The activities of CAT (129 18±7 6)U was lower than that of the control group (226 61±11 4)U. Differences were significant. However,in preventive groups,the blood lever of BUN,Cr,TG and TC were (13 2±0 2)mmol/L,(217 3±13 5)mmol/L,(1 82±0 63)mmol/L and (1 17±0 08)mmol/L,much lower than those of the diabetic group. Contrarily,the activity of CAT was (17 5±1 65)U markedly higher than that in the diabetic group. Pathological examination of kidney showed that diabetic rats enlarged glomerula,thickened basement membrane. Compared with the diabetic rats,glomerular enlargements of trial groups were inhibited in some degree. [Conclusion] TP has protective effects on kidney of diabetic rats to some degree.