上海市SARS可疑病例主要呼吸道病毒培养检测

Detection of commonly encountered virus in samples of severe acute respiratory syndrome probable case of shanghai

〔目的〕检测主要上呼吸道病毒 ,对上海市可疑SARS病例进行诊断排除。〔方法〕用MDCK、HEP - 2、LLC三种定量混合细胞分离培养以及荧光抗体染色检测标本中主要呼吸道病毒。〔结果〕上海市 2 4例SARS可疑病例检出甲 3型流感病毒 2例、乙型流感病毒 5例、呼吸道合胞病毒 1例、副流感病毒 1例。〔结论〕采用流行病学、临床表现进行初步诊断 ,SARS可疑病例中有一部分患者是由常见呼吸道病毒引起的。

睴bjective〕To detect main respiratory viruses and confirn the agents of SARS probable case.〔Methods〕 We inoculate throat swab specimens into a shell-vial of mixed cells of MDCK, HEP-2 and LLC cells and detect vires with monoclonal antibody-based immunofluorescence assay.〔Results〕 2 positive cases of A (H3) type influenza vires, 5 of B type influenza virus, 1 of respiratory syncytial virus and 1 of parainfluenza virus are found in 24 SARS probable cases in Shanghai.〔Conclusions〕 Through epidemiology and primary clinical diagnose, some of SARS probable cases are caused by common respiratory viruses.