透明质酸钠对兔颞下颌关节骨病的作用

The Effect of Sodium Hyaluronate on The Rabbit Osteoarthrosis

目的通过制动兔的颞下颌关节,制造骨关节病动物模型,采用组织病理学和组织化学方法研究透明质酸钠对制动的颞下颌关节的作用。方法普通家兔18只,分为2个实验组和1个对照组。实验组动物颞下颌关节制动后,左侧关节腔内注射0.06ml透明质酸钠,右侧关节腔内注射同等量的生理盐水,分别观察2周和4周后处死,完整取出双侧颞下颌关节(TMJ)作组织学及组织化学染色,评价关节软骨的改变及软骨中糖胺多糖含量的变化。结果与注射透明质酸钠的左侧关节相比,注射生理盐水的右侧TMJ出现了明显的退行性骨关节病的改变,两者具有显著性差异(P<0.01)。结论透明质酸钠对制动后TMJ的退行性变可起到明显的抑制作用。

Objective To study the effect of high-molecular-weight sodium hyaluronate on rabbit osteoarthrosis,which was made by immobilizing the temporomandibular joint.Methods18common rabbits were equally divided into2experi-mental groups and1control group.After the rabbit temporomandibular joint were immobilized,0.06ml high-molecular-weight sodium hyaluronate were injected into the left TMJs while the same amount of physiological Saline into the right ones.The animals were terminated after2weeks and4weeks respectively.The whole bilateral TMJs were removed and histopathological and histochemical examinations were performed to evaluate the changes of articular cartilage and the content of glycansaminoglycan in articular cartilage.Results There were severe osteoarthrotic changes in the right TMJs,whereas the changes in the left ones were slight.There was a significant difference between them(P<0.01).Conclusion High-molecular-weight sodium hyaluronate can restrain the degenerative changes of the immobilized TMJs.