新几内亚凤仙离体快速繁殖技术研究

Study on Rapid Propagation Techniques of Impatiens Hawkerii in Vitro Culture

取新几内亚凤仙具有顶芽或腋芽的新生茎段为外植体 ,诱导芽体萌发 ,进行繁殖培养 .通过大量试验 ,筛选出各阶段适宜的培养基组成 .萌芽诱导阶段培养在MS + 6-BA2 0mgL +NAA0 2mgL中 ,顶芽萌发早 ,生长快 ;在继代和增殖培养中以MS + 6-BA1 5mgL +NAA0 2mgL培养基效果最好 .1 2MS +NAA0 2mgL诱导生根 ,生根率可达 1 0 0 % ,根质量较好 ;适宜条件下炼苗移栽 ,试管苗成活率达 95

The new stem nodes of Impatiens hawkerii with terminal buds and axillary buds were taken as explant cultured in vitro to induce buds sprouting and multiplication. Media supplemented with different plant hormones and concentration of them were required for different stage of the culture. The medium for germination: MS+6 BA2.0mg/L+NAA0.2mg/L. Terminal buds of the explants sprouted early and grew fast in this medium. The medium for subculture and shoot multiplications: MS+6 BA1.5mg/L+NAA0.2mg/L, which was the first option. The medium for rooting:1/2MS+NAA0.2mg/L. The rooting rate was 100% and the quality of the roots grew well. The survival rate of the plantlet was 95% after transplanting under suitable condition.