草鱼淋巴器官中的肥大细胞

Mast cells in the lymphoid organs of Ctenopharyngodon idella

通过组织化学与形态学研究首次在草鱼的胸腺和头肾等淋巴器官中证实了肥大细胞的存在。草鱼肥大细胞的染色性质在某些方面似乎类似于哺乳动物的粘膜肥大细胞。Carnoy氏液及阿尔新蓝分别是优良的固定剂及染料,而中性缓冲甲醛却完全阻断草鱼肥大细胞的着染。值得注意的是一些曾被用于鉴定硬骨鱼肥大细胞(或嗜酸性颗粒细胞)的组织化学技术在本研究中未能检出草鱼肥大细胞。电镜下证实,草鱼肥大细胞中含有大量特征性的胞浆颗粒,其中充满无定形颗粒状基质,基质电子密度有的较高,有的较低,但未见特殊的亚微结构。

Mast cells were identified first in lymphoid organs including thymus, head kidney and the splenic sinus of grass fish (Ctenopharyngodon idella) histochemically and morphologically. It seemed that the staining properties of grass fish mast cells were similar to mammalian mucosal mast cells in some aspects. Carnoy's fluid and Alcian blue proved to be the good fixative and excellent dye for grass fish mast cell, respectively, but neutral buffered formalin (NBF) completely blocked the staining of the cells. It was noticed that some other histochemical methods previously used to detect mast cells, or so called eosinophilic granule cell (EGC) in teleostean fish, failed to identify grass fish mast cells in present study. Under electron microscopy a great deal of characteristic cytoplasmic granules were observed in the grass fish mast cells. The granules contained amorpholous granular matrix but electron density of the matrix from low to high. Special substructures were not observed in the cytogranules.