牛血清白蛋白制品中杂蛋白对牛血清ELISA的影响

The effect of impurity protein in BSA products on the ELISA of bovine serum

本试验对 8种不同厂家生产的牛血清白蛋白 (BSA)产品进行电泳试验和ELISA试验 ,判断BSA产品中杂质蛋白对ELISA检验 ,尤其是牛血清ELISA是否有影响。试验结果表明 ,(BSA)中含有杂质蛋白时 ,尤其是γ_球蛋白 ,会严重干扰牛血清ELISA结果。因此 ,在进行牛血清ELISA时 ,必须慎重选择BSA制品 ,以使ELISA检验结果更为准确。

Purity of eight kinds of Bovine serum albumin (BSA)products from different manufactures are examined by ELISA and electrophorsis.The result shows that the impurities which are included in the BSA products will interfere the result of the bovine serum.Therefore, it is important to choose the BSA products carefully,when we do the ELISA of bovine serum.