三种PCR方法诊断猪伪狂犬病的比较研究

Comparison of three PCR methods in diagnosis of porcine pseudorabies

针对伪狂犬病毒gD基因的不同片段设计三对引物 ,分别进行PCR扩增用于猪伪狂犬病的诊断 ,扩增的三个片段的长度分别为 2 62bp、2 1 7pb以及 1 2 0 3bp的全基因。通过比较发现 ,这三种PCR诊断方法均具有很高的特异性和敏感性 ,但扩增gD基因内部 2 62bp的PCR诊断方法种具有突出优点 ,其退火与延伸合成一步 ,其操作可于 1h内完成 ,敏感性更高 ,更适于猪伪狂犬病的快速诊断。

Three different fragments,262 bp, 217 bp lying PRV gD gene and the 1 203 bp integrated PRV gD gene were amplified by PCR using three pairs of primers for the diagnosis of pig pseudorabies. Through comparison of the three PCR methods,it was found that they provided excellent specificity and sensitivity to diagnosis of pseudorabies and the amplification of 262 bp fragment could be used for rapid diagnosis of PRV infection in pigs making annealing-elongation on the same temperature, taking less than l hour to complete.