气相色谱法测定健康人含服速效救心丸后冰片的血药浓度

Determination of Borneol in Blood After Keeping Suxiao Jiuxin Pills in Healthy People's Mouth by GC

目的 :用气相色谱法测定健康人含服速效救心丸后冰片 (龙脑、异龙脑)的血药浓度。方法 :采用HP -5石英弹性毛细管柱 ,柱温为85℃ ,汽化室温度为180℃ ,检测器温度为200℃ ,载气为氮气 ,萘为内标。结果 :冰片进样量在50～1000ng/ml范围内其峰面积与进样量呈良好线性关系 ,Y=—0 1273+0 0015X ,回收率为 (98 7±2 31) % ,RSD=2 76% (n=6)。健康人血清中冰片含量在15min左右达血药浓度高峰。结论 :建立的气相色谱法可靠、灵敏、简便 。

OBJECTIVE:To determine the concentration of borneol in blood after keeping Suxiao Jiuxin pills in healthy men's mouth by GC METHODS:HP-5 quartz elasticity capillary column(15m×0 53mmID) was used with nitrogen as the carrier gas,temperature of column was 85℃ The injector temperature and detector temperature were 180℃,200℃ respectively Naphthalene was used as internal standard RESULTS:The standard curve was linear within the concentration range of 50～1 000ng/ml of borneol,the calibrating equation was Y=-0 1 273+0 0 015X The recovery was (98 7±2 31)%,(RSD=2 76%,n=6) The peak of blood drug concentration was obtained in 15 min or so CONCLUSION:The method was simple,rapid,accurate and suitable for pharmacokinetic study of preparation containing borneol