信号变换-多元校正方法对复方甲硝唑注射液的分析

Analysis of Co. Metrozole Injection by the Signal Conversion Combined with Multicomponent Calibration Methods

目的 :采用信号变换 -多元校正方法对复方制剂进行分析。方法 :通过一阶导数 (D1)变换获取导数光谱数据 ,结合应用直接校正法即多元线性回归法 (MLR)与间接校正法即偏最小二乘法 (PLS) ,测定甲硝唑和氯霉素的含量。结果 :用D1-MLR法和D1-PLS法测定样品中两组分的标示百分含量和相对标准偏差 ,D1-MLR法测定甲硝唑的结果分别为99 6%、0 11 % ,氯霉素分别为99 3 %、0 20% ;D1-PLS法测定甲硝唑的结果分别为97 5 %、0 43 % ,氯霉素分别为94 6 %、0 89 %。结论 :一阶导数法应用于直接校正法效果比用于间接校正方法好。

OBJECTIVE:To analyse compound pharmaceutical preparations by the signal conversion combined with multicomponent calibration methods METHODS:One-order derivative spectrum data was obtained by one-order derivative conversion On the basis of this,we combined them with the MLR method and PLS method to determine the contents of metrozole and chloromycetin in co metrozole injection RESULTS:The labelling quantity and RSD(n=5) were:metrozole 99 6%,0 11%;chloromycetin 99 3%,0 20% by derivate-MLR;The labelling quantity and RSD(n=5) were:metrozole 97 5%,0 43%;chloromycetin 94 6%,0 89% by derivate-PLS respectively CONCLUSION:One-order derivative spectrum signals aplied to the MLR method is better than the D1 PLS method