摘要
以甜樱桃矮化砧木Gisela5和Gisela6作为试材,研究优化了其离体快繁的条件.结果表明:提高基本培养基中氮素含量可提高增殖系数,含有NH4NO33300mg/L和KNO33800mg/L的MS基本培养基中添加BA0.5mg/L和IBA0.1mg/L的培养基,为Gisela5和Gisela6的最适增殖培养基,其增殖系数可达7-8.在1/2MS+IBA0.3mg/L的生根培养基上,生根率达89.3%,每个外植体可生出3-4条根.生根苗移栽容易成活.
Stem explants and shoot tips obtained from a mature tree of cherry rootstock, Gisela5 and Gisela6(Prunus cerasus L.×P. canescens L.), were successfully established in vitro, and they were easing micro propagated on MS medium. As the nitrogen concentration in MS medium increase the shoot number increase too. The modified MS medium containing 3800mg/L potassium nitrate, 2745mg/L ammonium nitrate, 0.5mg/L benzyladenine and 0.1mg/L indolebutyric acid was optimal to form multiple shoot. After 10 times successive subcultures, 7-8 shoots per explant were produced. Shoot segment developed adventitious roots within 20 days in 1/2 strength MS medium containing 0.3mg/L indolebutyric acid. Eighty percent of the shoots rooted were successfully transplanted into greenhouse.
出处
《安徽师范大学学报（自然科学版）》
CAS
2003年第2期161-164,共4页
Journal of Anhui Normal University(Natural Science)
基金
SupportedbytheMinistryofAgricultureofShandongProvince(2000-3009)
CouncilofScienceandtechnologyofShandongProvince(99010306) .
