摘要
目的 :了解不同种类葡萄球菌在本院临床标本中的感染情况及耐药性 ,为临床治疗和控制此类感染提供参考。方法 :采用VITEK AMSGPI卡进行鉴定 ,纸片扩散法 (NCCLS)及GPS卡进行药敏实验、β内酰胺酶测定和MRS检测 ,并结合临床资料进行分析。结果 :总共 375 3株感染菌中 82 2株为葡萄球菌 (2 1.90 % ) ,其中金黄色葡萄球菌 (SA) 32 2株 (39.17% ) ,凝固酶阴性葡萄球菌 (CNS) 5 0 0株 (6 0 .83% )。SA中 2 93株 (90 .99% )产生 β内酰胺酶 ,苯唑西林耐药者 (MRSA) 183株(5 6 .83% ) ;CNS中 4 5 0株 (90 .0 0 % )产生 β内酰胺酶 ,苯唑西林耐药的凝固酶阴性葡萄球菌 (MRCNS) 35 0株 (70 .0 0 % )。 结论 :葡萄球菌特别是凝固酶阴性葡萄球菌是临床感染的主要致病菌之一 ;万古霉素是有效治疗苯唑西林耐药的葡萄球菌(MRS)的唯一可选药物 ;合理使用抗生素对治疗和控制感染至关重要。
Objective:To study the role of various species of Staphylococci in the clinical infections and their drug resistance with a view to offering references for the control of the infection.Methods:VITEK AMS GPI card was used for identification,disc diffusion test methods(NCCLS)and GPS card were used to detect the drug resistance,beta lactamase and MRS.Results:Among the 3 753 strains isolated from clinical samples,822( 21.90 %) strains were staphylococci ,among which 322 strains(39.17%) were staphylococci aureur (SA) and 500(60.83%) coagulase negtive staphylococci (CNS).90.99%(293) of SA produced beta lactamase and 56.83 %(183) were MRSA;90.00% of CNS were beta lactamase positive strains and 70.00% were MRCNS.Conclusions: Staphylococci, especially CNS,is one of the main pathogens leading to clinical infections.Vancomycin is the only available effective antibiotic.It is very important to use antibiotics properly for the control of the infections.
出处
《蚌埠医学院学报》
CAS
2003年第4期358-360,共3页
Journal of Bengbu Medical College
基金
安徽省十五攻关基金资助 ( 0 10 13 0 3 9)
关键词
葡萄球菌
耐药性
细菌学技术
staphylococcus
drug resistance
bacteriological technics