佐剂性关节炎大鼠滑膜巨噬细胞在关节破坏中的作用

Role of synovium macrophage during adjuvant arthritis in rat articular damage

目的 :了解佐剂性关节炎 (AA)大鼠滑膜巨噬细胞在关节破坏中的作用。方法 :采用大鼠尾部皮内注射完全福氏佐剂建立AA模型 ,不同时点处死后后肢关节X线照片并进行放射学指数 (RI)评分 ,膝关节滑膜石蜡切片HE染色和ED1免疫组化染色及计数 ,分析RI与滑膜衬里层细胞层数及ED+ 1细胞数的相关性。结果 :AA大鼠滑膜衬里层细胞层数、衬里层及衬里下层ED+ 1细胞数与RI水平呈显著正相关。结论

AIM: To explore the role of synovium macrophage of adjuvant arthritis (AA) rats in articular damage. METHODS: AA was induced in female Wistar rats by means of intradermal injection with complete Freud's adjuvant. X-ray on hind paws of AA rats was took at different time points and radiograph index (RI) was then evaluated. Synovium macrophages were detected through immunohistochemistry with ED 1 monoclonal antibody. The correlations between RI and synovial lining layer count/synovium macrophage count were also analysed. RESULTS: Synovial lining layer count, both macrophage count in synovial lining layer and sublining layer were positively correlated with RI. CONCLUSION: Synovium macrophages of AA rats may play an important role in articular damage.