小鼠眼内移植三种B16黑色素瘤细胞系的比较研究

Comparative study on intraocular transplatation of three B16 melanoma cell lines in mice

目的 :比较B16F0、B16F1、B16F10 3种黑色素瘤细胞系的细胞移植于该肿瘤细胞系来源的C5 7BL/6小鼠前房内的进展情况。方法 :将此三种黑色素瘤细胞系培养后的活细胞悬液移植于C5 7BL/6小鼠前房内 ,观察并比较其眼球溃破时间、淋巴结和肺的转移及生存期。结果 :F10组移植肿瘤眼眼球溃破时间早于另两组 ,而F0组与F1组间无差异。在鼠前房移植肿瘤细胞后的 18d ,F1组及F10组鼠出现移植肿瘤眼同侧的颈部淋巴结转移 ,而F0组未出现类似情况 ;在鼠前房移植肿瘤细胞后的 2 8d ,3组均出现肺转移。F0组小鼠生存时间长于另两组 ,而F1组与F10组间无差异。结论 :3种黑色素瘤细胞系的细胞移植于小鼠前房内后进展不同 。

AIM: To establish an animal model for studying the development and metastasis of melanoma. METHODS: C57BL/6 mice were used as host to receive melanoma cell transplantation. Three kinds of melanoma cell lines, B16F0, B16F1 and B16F10, cultured to prepare the cell suspensions, were transplanted into the mouse anterior chamber (AC) of the eye. The time of eyeball diabross, time of survival and metastasis of lymph node and lung were observed. RESULTS: The time of eyeball diabross in F10 group was earlier than that in other groups. The time of eyeball diabross was no difference between F0 and F1 groups. Metastasis was developed 18 days after transplantation in F1 and F10 groups, where the tumor cells was found in ipsilateral cervical lymph nodes. The melanoma cells metastasized to lung in all three groups 28 days after transplantation. The survival time in F0 group was longer than F1 and F10 groups. There was no difference in survival times between F1 and F10 group. CONCLUSION: The differences of three kinds of melanoma cell lines in tumor development and metastasis provided the evidence that was useful for choosing suitable animal model further to study the eye melanima.