摘要
为揭示分布于西鄂尔多斯高原的中国特有种四合木对生境的适应性及与其他属的亲缘关系,用PCR直接测序方法,对四合木与同生境蒺藜科(Zygophllyaceae)其他3个种的核糖体DNAITS片段进行了序列分析.结果表明,4种植物在ITS片段长度、GC百分含量和变异位点数上均存在一定差异.ITS1长177~212bp,ITS2长218~237bp;GC含量为57.7%~62%,信息位点比例30.1%.系统发育分析表明,四合木(TetraenamongolicaMaxim)与霸王(ZygophyllumxanthoxylumMaxim)在系统位置上较相近,白刺(NitrariasibiricaPall)与蒺藜(TribulusterrestrisL.)较相近.ITS所揭示的亲缘关系与这些种的形态学和胚胎学特征相似.
The phylogenetic relationships between T.mongolica and other three species of Zygophyllaceae were analyzed based on their nucleotide sequences of the internal transcribed spacer (ITS) of the 18-26s nuclear ribosomal DNA.The sequences were obtained by PCR technique.The results showed that there were differences in the length of sequences,contents of G+C and numbers of variable site among the four species. The length of ITS1 sequences ranged from 177 to 212 bp and ITS2 from 218 to 237 bp,The fragments of ITS2 were longer than that of ITS1.The contents of G+C ranged from 57.7% to 62%.Phylogenetic analysis indicated showed that T. mongolica was close to Z. Xanthoxylum in systematic position.Nitraria sibirica and Tribulus terrestris almost same in the fragments of ITS2, but different in ITS1.The results were similar to the achievements in the research of morphology and anatomy.It reflected their universality and peculiarity of evolutionary process to fit the environment.
出处
《内蒙古大学学报(自然科学版)》
CAS
CSCD
北大核心
2003年第4期420-424,共5页
Journal of Inner Mongolia University:Natural Science Edition
基金
国家重点基础研究发展规划(G2000048704)
国家自然科学基金项目(30160020)
关键词
nrDNAITS
四合木
霸王
白刺
蒺藜
系统位置
nrDNA ITS
Tetraena mongolica
Zygophyllum xanthoxylum
Nitraria sibirica
Tribulus terrestris
systematic position