不同品系小鼠自发活动特点及中枢抑制药新评价模型

A New Model for Evaluating Locomotor Activity and Central Depressive Drugs in Different Strain Mice

目的 用开场实验研究NIH、ICR、昆明种小鼠自发活动特点,并以戊巴比妥为工具药建立药物中枢抑制作用新的评价模型.方法 用开场实验观察小鼠进入新环境的自发活动特点,并用计算机图像处理系统记录,分析小鼠30min内的活动轨迹,观察不同时间段的总路程、中央逗留时间、中央路程3个参数.还观察了腹腔注射不同剂量戊巴比妥对自发活动特点的影响.结果 各种小鼠总路程在前10min明显大于后20min(P<0.001);中央逗留时间和中央路程变化较小,但存在一定的品系和昼夜差异.戊巴比妥可减少ICR和NIH小鼠(昆明种小鼠无效)的总路程,但无典型的量效关系;但可剂量依赖性减少各种小鼠中央逗留时间和中央路程(P<0.01).结论 本实验建立的方法能反映小鼠自发活动特点,并可用于评价中枢抑制药作用,其中中央逗留时间和中央路程是稳定、敏感的指标.

ObjectiveTo establish a new model for evaluating locomotor and central depressive drugs in NIH, ICR, Kunming (KM) mice.MethodsLocomotor activities in an open field box were observed, and the locomotor tracks within 30 min were recorded and analyzed by a computer imaging analyzer. The indexes used were total distance, the ratio of central time/total time, and the ratio of central distance/total distance. Pentobarbital was used as model drug, and its effects on locomotor properties were analyzed after ip injection at 7.5, 15 and 30 mg·kg-1. ResultsIn the three strains of mice, the total distance within the first 10 min was longer than those of the second and third 10 min durations (P < 0.01); and the difference of the activities in central region was small but showed some strain and circadian differences. Pentobarbital reduced the total distance in ICR and NIH (not in KM) mice, but showed no remarkable dosedependency. However, pentobarbital reduced the ratios both central time and central distance dosedependently in the three strains of mice. ConclusionThe model established in this study is useful to evaluate the properties of locomotor activity and the effects of central depressive drug. Among these indexes, the ratios for the activities in the central region are more stable and sensitive.