象山地区中国对虾白斑综合征病毒超微结构观察

Ultrastructure of Xiangshan Isolate of White Spot Disease Virus of Penaeus chinensis

从患病的中国对虾(Penaeuschinensis)头胸部分离提纯出一种杆状病毒,负染观察发现,病毒粒子的核衣壳呈杆状,大小约为(250～290)nm×(65～80)nm,每圈螺旋宽约20nm,核衣壳的两个末端之间一般有13～15圈衣壳螺旋.个别病毒有较长的核衣壳,螺旋数达30圈,长度达到600nm.有部分病毒核衣壳的一端有30～65nm的凹陷.还有部分病毒核衣壳一端有30～60nm的突出部分.

A high virulent of white spot disease virus has been got in Xiangshan, Zhejiang Province.Most nucleocapsid,observed by negative stain,was rodshaped,and ranged in the size of 250～290nm in length and 65～80nm in breadth respectively.The viral nucleocapsid was a spiral cylinder with 15 cycles.Three nucleocapsid with different structure were also observed occasionally:①Some nucleocapsid with 30 cycles;②Some nucleocapsid with 30～65nm incisure;③Some nucleocapsid has 30～60nm protrusion portion on one end.