摘要
通过不同浓度的NaCl、NaI修饰硅纳米颗粒 ,用琼脂糖凝胶电泳分析硅纳米颗粒与DNA结合力及对DNA的保护作用 ,同时用绿色荧光蛋白基因作报告基因 ,以硅纳米颗粒作为基因转染的载体 ,转染HT10 80细胞。经电镜观察证实硅纳米颗粒进入细胞内 ;硅纳米颗粒与DNA结合后 ,能对DNA起保护作用 ;并且硅颗粒作为基因转染的载体 ,将绿色荧光蛋白基因导入HT10 80细胞 ,用荧光显微镜观察到发绿色荧光的细胞。结果表明 。
The silica nanoparticles are modified by sodium iodine or sodium chloride with different concentration.Their ability to bind and protect plasmid DNA was demonstrated by agarose gel electrophoresis.The EGFP N1 plasmid was transfected into HT1080 by combing silica nanoparticle.Eletromicroscope examine revealed that nanoparticle DNA could enter into cells,these silica nanoparticles protect DNA against nuclease digestion and the GFP has an effective expression.In conclusion,these nanoparticles might be used as DNA carriers for gene transfection.
基金
国家自然科学基金资助 (编号 :3 983 0 2 0 0 )~~
关键词
硅纳米颗粒
氯化纳
基因转染
载体
silica nanoparticle
sodium chloride
gene transfection
carrier
