莱茵衣藻(Chlamydomonas reinhardtii)nfr基因的显隐性突变性质及其与叶绿体psbA基因之间的相互作用分析

Analysis of Dominant-recessive Mutated Character of nfr Genes of Chlamydomonas reinhardtii and Its Mutual Interaction with the Chloroplastic psbA Gene

通过对莱茵衣藻 (Chlamydomonasreinhardtii)nfr/Nfr杂合二倍体的表型分析证明 ,nfr基因是隐性突变基因 ,Nfr 4和Nfr 5突变株对达草灭的抗性是由nfr 1和nfr 2两个不同核基因的隐性突变所导致。psbA基因突变株品系与野生型品系CC 12 4和nfr基因突变株进行杂交并对其后代进行的四分子分析结果表明 :在光养条件下 ,叶绿体psbA基因突变株品系对达草灭的敏感性是psbA突变等位基因的多效效应 ;而在混合营养条件下 ,叶绿体基因组对达草灭抗性性状也产生一定影响。达草灭抗性突变株品系对抗菌素类的交叉抗性性质进行的检测实验结果中发现 ,Nfr 3对红霉素和链霉素具有一定的交叉抗性 ,预测 。

Phenotypic analysis of the nfr/Nfr diplontic hybrids has demonstrated the recessive mutated character of the nfr genes ,and the norflurazon resistant character of the Nfr 4 And Nfr 5 mutants were resulted in by the different nuclear genes nfr 1 and nfr 2 respectively.From the tetrad analysis result of hybridizations of psbA mutant with wild strains CC 124 and nfr mutant,it have been demonstrated that the sensitivity of psbA mutated strain to norflurazon under photoautotrophic condition is due to its multiple effect whereas the chloroplast genome under the mixotrophic situation also gives some effect for resistance to norflurazon.From the titration results of cross resistant character for antibiotics of norflurazon resistant strains,we had found that,the Nfr 3 mutant has some cross resistance to erythromycin and streptomycin;based on this phenomenon it would be expected that this mutant having resistant character to suppressor of phytoene desaturase also give some effect for construction of chloroplastic protein.