摘要
由 19头杂种公猪 [皮特兰× (皮特兰×汉普夏 ) ]、5 2头杂种母猪 [Leicoma× (大约克×长白 ) ]及其 332头后代组成的商品群作为参考系 ,选择 172个微卫星标记和 3个Ⅰ类标记 (RYR1、PIT1、PRKAG3)对参考系的个体进行遗传标记分型 ,应用CRIMAP(2 4 )构建猪的整个基因组微卫星连锁图谱。采用多重PCR方法对微卫星标记进行扩增 ,用ABI 377测序仪进行电泳分离 ,应用Genescan 3 0和Genotyper 2 0软件进行基因型检测。 3个Ⅰ类标记用PCR RFLP技术进行分型。CRIMAP程序分析表明 :所构建的猪常染色体性平均连锁图谱的总长度为 2 36 8 7cM ,X染色体的长度为 14 3 10cM ,遗传标记的平均间距为 16 3cM ,亲本的微卫星标记座位的杂合度平均为 0 70。此连锁图谱的构建将为商品猪群的生长、胴体、肉质以及繁殖性状的QTL扫描打下基础。
A commercial population created with 19 hybrid boars [Piétrain×(Piétrain×Hampshire)],52 hybrid sows [Leicoma×(Large White×Landrace)] and their 332 offspring was used to construct a whole genome porcine linkage map.The genetic markers used in this study include 172 microsatellite markers and 3 type Ⅰ markers ( RYR1?PIT1 and PRKAG3 ).All microsatellite markers were genotyped using multiplex PCR reactions and visualized on ABI 377 sequencer.Three type I markers were assayed using PCR RFLP technique.CRIMAP (2 4) analysis revealed a total length of the sex averaged map for SSC1～SSC18 by 2449 2 cM and the length of SSCX by 143 1 cM.The average interval distance accounts to 16 3 cM.The heterozygosity of the parents at the microsatellite loci averaged 0 70.This map will play an important role in screening of QTL for growth,carcass and meat quality and reproduction in commercial populations.
基金
德国H .WILHELMSCHAUMANN基金会资助~~
关键词
猪
连锁图谱
微卫星标记
pig
linkage map
microsatellite marker
