大鼠Y-型迷宫测试指标正常值的确定

The normal values of the testing indexes of Y-type maze test in rats

目的 确定不同年龄段雄性SD大白鼠明暗辨别性条件反射和一次性被动回避反应各项指标的正常值 ,为基础和临床有关动物学习和记忆能力的实验研究提供参考。方法 采用一种相对简便可靠的“随机不休息法”对不同年龄段雄性SD大白鼠进行Y -型迷宫测试。结果 1月龄、2月龄、4月龄、12月龄、2 4月龄大鼠的EN ;TRT ;Lst ;Lsd的正常值分别为 4.7± 1.2次、1.4± 1.14次、1.62± 0 .5 5次、1.2 5± 0 .7次、3 .8± 1.0 5次 ;12 5 .5± 42 .0 1s、117.8± 2 5 .0 4s、93 .2 2± 18.2 7s、10 9.5 8± 5 .2 7s、161.46± 4.46s ;2 2 .3 9± 8.46s、2 3 .2 5± 6.6s、17.83± 12 .3s、18.83± 13 .42s、2 8.83± 13 .42 ;2 3 .94± 9.0 5s、2 0 1.3± 3 9.1s、2 15 .3 3± 45 .4s、198.6±3 2 .9s、13 4.2± 3 7.2。结论 正常幼年大鼠和老年大鼠的学习记忆能力较差 ,正常成年大鼠学习记忆能力较强。

Objective In order to offer references for the basic and clinical studies on animal's abilities of learning and memory, the normal value of the indexes of the one trial passive avoidance response (Step through test) and bright dark discrimination conditional reflex were established. Methods Using “random restless” testing method (a kind of relatively simple and reliable method of Y type maze) to test the learning and memory's ability of male SD rats in different age stages. Results The normal values of EN;TRT;Lst and Lsd in 1 month age rats、2 months age rats 、4 months age rats、12 months age rats and 24 months age rats respectively are: 4.7±1.2 times、1.4±1.14 times、 1.62±0.55 times、 1.25±0.7 times、 3.8±1.05 times;125.5±42.01s、 117.8±25.04 s、 93.22±18.27 s、109.58±5.27 s、 161.46±4.46 s;22.39±8.46 s、 23.25±6.6 s、17.83±12.3 s、18.83±13.42 s、28.83± 13.42 ;23.94±9.05 s、201.3±39.1 s、 215.33±45.4 s、 198.6±32.9 s、134.2±37.2. Conclusion The abilities of learning and memory in infant rats and aged rats are poorer and the adult rats' abilities of learning and memory are stronger.