400例泌尿生殖道支原体、衣原体检测及药敏结果分析

Analysis of Detection and Susceptibility Results for 400 Ureaplasma and Chlamydia Strains from Genital-urinary Tract

目的 了解泌尿生殖道支原体、衣原体的感染状况 ,以及解脲脲支原体对不同药物的体外敏感性。方法 沙眼衣原体检测采用美国 ABI公司 Sure Step TM Chlamydia抗原检测卡 ;支原体培养试剂盒采用上海奥普生物医药有限公司产品 ;支原体培养、药敏一体试剂盒采用珠海浪峰生物技术有限公司产品。结果 4 0 0例样本中解脲脲支原体 (U u)阳性 134例占 33.5 % ;人型支原体 (Mh)阳性 4 4例占 11.0 % ;沙眼衣原体 (CT)阳性35例占 8.75 %。对 96株解脲脲支原体敏感率 >89%的药物有多西环素 (强力霉素 )、米诺环素 (美满霉素 )、交沙霉素和阿奇霉素。结论 Mh的分离率位于第 2 ,若常规检测只做单项 Uu,将势必造成一定的漏检 ,U u对常见的红霉素、四环素等已产生一定的耐药率 ,及时进行药敏试验、选择高效药物、实施足剂量和足疗程用药 ,对降低 U u耐药株的产生、防止复发和提高治愈率将起到重要作用。

OBJECTIVE Investigation of Mycoplasma and Chlamydia from genital urinary tract specimens and susceptibility of Ureaplasma urealyticum(Uu) strains to antimicrobial agents. METHODS The Sure Step TM Chlamydia kit was used for detection of Chlamydia trachomatis, Ureaplasma culture kit from Shanghai OUPU Biomedical Co. Ltd. for isolation of Ureaplasma. Combination of Ureaplasma culture and susceptibility testing kit from Zhuhai Langfeng Biotech Co. Ltd. for isolation and susceptibility testting of Ureaplasma. RESULTS For 400 specimens, there were 134 Uu strains(33.5%), 44 Mycoplasma hominis strains(Mh, 11.0%) and 35 Chlamydia trachomatis strains(8.75%). Nity six Uu strains were more than 89% susceptible to doxycyline, josamycin, azithromycin and minomycin. CONCLUSIONS The Mh was the second most common pathogens in genital urinary tract specimens. So the pathogens in genital urinary tract will be underestimated if only the Uu is detected. The Uu has been resistant to common use antibiotics such as erythromycin and tetracycline. It is important for doctors to use susceptibility testing results to select efective drugs and reduce resistance.