1633例患儿标本微生物培养结果及耐药性分析

Culture Results and Resistance of Microorganism: An Analysis of 1 633 Samples in Children

目的 通过对病原微生物感染分布和细菌耐药性分析 ,为临床选用抗生素提供科学、合理、可靠依据。方法 对我院 16 33例临床标本进行普通培养和高渗培养 ,以 K- B法进行药敏分析。结果 血、痰、咽拭子、脑脊液、尿标本细菌培养 ,阳性率分别为 16 .5 %、6 5 .6 %、4 0 %、14 .3%、6 5 .5 % ,支原体培养阳性率 17.3% ;革兰阳性菌对青霉素、苯唑西林、头孢他啶的耐药率 >5 0 % ;革兰阴性菌对氨苄西林、羧苄西林、林可霉素的耐药率 >80 %。结论 加强病原微生物培养及耐药性的定期系统监测 ,对临床合理使用抗生素 ,预防医院感染具有重要意义。

OBJECTIVE To provide scientific, reasonable and reliable evidence to the clinicians by analysing the infected distribution of microorganism and the resistance of bacteria. METHODS A total of 1 633 samples were cultured in ordinary and hyperosmotic agars, and analysed drug sensitivity by Kirby Bauer. RESULTS The positive rate from blood, sputum, pharyngorrhea, CSF, and urine culture was 16.5%, 65.6%, 40%, 14.3% and 65.5%, respectively. Mycoplasma from sputum was 17.3%. The resistant rates of Gram positive bacteria to penicillin, oxacillin and ceftazidime were more than 50%. The resistant rates of Gram negtive ones to ampicillin, carbenicillin and lincomycin were more than 80%. CONCLUSIONS It′s significant to instruct clinicians using antibiotics reasonably and prevent nosocomial infection by detecting the culture and resistance of microorganism systematically.