摘要
目的:研究预电刺激小脑顶核对大鼠脑缺血再灌注后Ku70 mRNA的表达及其神经保护的分子机制。方法:Wistar大鼠通过原位杂交的方法及末端标记法检测Ku70 mRNA的表达及Tunel阳性细胞数。结果:①单纯造模组及毁损小脑顶核组缺血/再灌注后各时点Ku70 mRNA的表达无显著性差异,均较预刺激组及假手术组明显减少(P<0.01),预刺激组除缺血/再灌注后6h Ku70mRNAR的表达较假手术组减少(P<0.01)外,余时点与假手术组Ku70 mRNA表达无明显差异;②预刺激组Tunel阳性细胞数较未给预电刺激的两组明显减少(P<0.01)。结论:①预电刺激小脑顶核能减少缺血区神经元凋亡可能与DNA修复酶Ku70活性上调有关;②毁损小脑顶核后电刺激对脑缺血/再灌注引起的氧化性DNA损伤无保护作用。
Aim: To investigate the molecular mechanisms of fastigial nucleus (FN) electrical stimulation protecting against ischemia-reperfusion induced oxidative DNA damage in rat brain. Methods: Ku70 mRNA was hybridized with a rat Ku70 cDNA probe. DNA fragmentation was detected by terminal deoxynucleotidyl transferase-mediated uridine 5'-triphosphate-biotin nick end-labeling(TUNEL) . Results :①Ku70 mRNA expression in pure model group after reperfusion was not different from that in FN-lesion group, but there was a significant reduction compared with FN-stimulation group and sham-operated group. ②The number of Tunel-positive cells in FN-stimulation group reduced significantly compared with that of pure model group and FN-lesion group ( P < 0.01). The number of Tunel-positive cells in pure model group was not different from that in FN-lesion group. Conclusion :①The neuroprotective mechanism of fastigial nucleus electrical stimulation is associated with upregulation DNA repair enzymatic activity, ②fastigial nucleus electrical stimulation does not protect against ichemia-reperfusion induced oxidative DNA damage in rat brain after fastigial nucleus-lesion.
出处
《中国临床神经科学》
2003年第2期117-120,共4页
Chinese Journal of Clinical Neurosciences
基金
国家自然科学基金资助项目(39370261)
