重症肌无力患者CD40配体高表达及其临床意义

Icreased Expression and Significance of CD40 Ligand on Peripheral Blood Mononuclear Cell in Patients with Myasthenia Gravis

目的:研究重症肌无力(MG)患者CD40配体(CD40L)的表达,探讨CD40L在MG中的作用。方法:25例MG患者,分为急性期组13例,非急性期组12例,设正常对照组15例。分离血中单个核细胞,分别培养并用不同刺激剂(PHA、PMA和A23187)刺激。培养前后用流式细胞仪检测CD40L阳性细胞率。结果:①MG急性期组CD40L阳性细胞率比正常对照组及非急性期组显著增高(均为P<0.01);②经PHA或PMA和A23187刺激后,非急性期组CD40L阳性细胞率也比正常对照组显著增高(P<0.01)。结论:CD40L的高表达与MG发生和加重有关。

Aim: To investigate the role of CD40 and CD40 lignad in the pathogenesis of myasthenia gravis (MG) , the CD40L expression on the peripheral blood mononuclear cells of patients with MG was tested. Methods: 25 patients with MG included 13 acute patients and 12 non-acute patients. 15 healthy people were acted as control group. Blood of patients and normal controls was collected. Mononuclear cells in peripheral blood (PBMC) were isolated and cultured in the medium (RPMI-1640) supplemented with PHA or PMA and A23187. After 6 hours, cell smples were collected and DL40L expression was tested by flow cytometry. Results: ①CD40L expression on PBMC in patients with acute MG was increased markedly compared with that of patients with non-acute MG and normal controls before activation, but there were no significantly statistics differences between patients with non-acute MG and normal controls. ②CD40L expression of patients with acute MG and non-acute MG were increased significantly in contrast to that of normal controls after activation with PHA or PMA and A23187,CD40L expression of patients with acute MG still increased markedly compared with that of patients with non-acute MG. Conclusion: Hyperexpression of CD40L is correlated with the clinical progression of MG.