差示分光光度法测定红霉素酒精中红霉素的含量

Determination of erythromycin in erythromycin tincture with difference spectrophotometry

目的 建立红霉素酒精中红霉素含量的测定方法。方法 利用红霉素在碱性条件下反应产物的紫外吸收特性 ,采用差示分光光度法测定红霉素酒精中的红霉素含量。测定波长为 2 3 5nm。结果 红霉素在 8～ 80mg·L- 1 浓度范围内与吸收度呈良好的线性关系。高、中、低三个浓度的平均回收率分别是 1 0 0 .4%(RSD =0 .6 7%)、1 0 1 .5 %(RSD =0 .43 %)和 99.5 %(RSD =0 .75 %)。结论 此法快速、简便、准确 ,适用于医院制剂分析。

Objective To establish a method for determination of erythromycin in erythromycin tincture. Methods The difference spectrophotometry was used for the determination and the detection wavelength was 235nm.Results Linear ranges were from 8 mg·L -1 to 80 mg·L -1 (r=0.9995).The average recoveries were 100.4%(RSD=0.67%),101.5%(RSD=0.43%) and 99.51% (RSD=0.75%) respectively.Conclusions The method is conveniont, rapicl and accurate. It is suitable to the analysis of hospital preparation