人胃癌原代细胞培养化疗药物敏感性评价

Evaluation of chemosensitivity of seven antitumor drugs commonly used for human gastric cancer with primary cul ture cells

目的:评价人胃癌原代细胞对7种化疗药物的敏感性。方法:取59例手术切除的新鲜胃癌组织标本,应用人胃癌原代细胞短期培养(混杂非肿瘤细胞)与纯化培养MTT法体外药敏试验,测定每种化疗药物对肿瘤细胞的抑制率。结果:药敏试验测定7种化疗药物抑制率除氨甲蝶呤(MTX)外纯化法均高于短期法,表明纯化法较敏感;两种方法测定药物敏感性顺序一致,由高至低依次为顺铂(DDP)、丝裂霉素(MMC)、阿霉素(ADM)、依托泊甙(VP-16)、5-氟尿嘧啶(5-FU)、羟基喜树碱(HCPT)、MTX。结论:7种化疗药物以DDP、MMC、ADM较敏感,VP-16、5-FU次之,HCPT、MTX最差。

Objective:To investigate the chemosensitivity of seven antitumor drugs commonly used in the treatment of gastric cancer with primary culture tumor cells.Methods :Fifty-nine fresh samples from patients with gastric cancer were obtained during operation.Chemosensitivity results from MTT assay with short-term culture tumor cells contaminated by nonmalignant cells were compared with those from the same assay with purified primary culture tumor cells.Results:All of the seven antitu-mor drugs but Methotrexate(MTX)using purified cells showed higher inhibition rates than those using short-term method,but the order of drugs in the degree of chemosensitivity was the same.They were(from high to low):Cisplatin(DDP),Mitomycin C(MMC),Adriamycin(ADM),Etoposide(VP-16),5-Fluo-rouracil(5-FU),Hydroxycampothecin (HCPT),MTX.Conclusions :DDP,MMC and ADM are more chemosensitive than VP-16or5-FU,especially than HCPT or MTX for gastric cancer.