山东省急性淋巴细胞白血病患儿HLA-A、B、DRB1等位基因多态性研究

The polymorphism study of HLA-A,B,DRB1alleles in children with acute lymphoblastic leukemia in Shandong Province

目的:研究山东省汉族急性淋巴细胞性白血病(ALL)患儿与等位基因HLA-A、B、DRB1的多态性的遗传关联。方法:采用聚合酶链反应-序列特异性寡核苷酸探针(PCR-SSO)方法,对197例ALL患儿和5841例健康脐带血样本等位基因HLA-A、B、DRB1多态性进行研究。结果:在等位基因HLA-A、B、DRB1中,ALL患儿的A11和A24;B40、B15、B56、B67和B27;DR9、DR12、DR14和DR16等基因的基因频率显著高于正常人群(P<0.01)。而HLA-B48、DR7和DR15等基因的基因频率显著下降(P<0.05)。结论:基因HLA-A11、A24、B40、B15、B56、B67、B27、DR9、DR12、DR14、DR16对ALL患儿有遗传易感作用,尤其是B40与ALL具有强相关性;而基因HLA-B48、DR7、DR15等对ALL患儿有遗传拮抗作用。

Objective:To study the genetic susceptibility of HLA-A,B,DRB1alleles in chil-dren with acute lymphoblastic leukemia(ALL).Method:HLA-A,B,DRB1alleles polymorphism in197children patients with ALL and5841normal umbilical cord blood controls were determined by PCR with sequence specific olignucleotide probe(PCR-SSO).Results:The allele frequencies of A11,A24,B40,B15,B56,B67,B27,DR9,DR12,DR14,DR16were increased(P<0.01),while the allele frequencies of HLA-B48,DR7,DR15were decreased in patients with ALL(P<0.05).Conclusion:HLA-A11,A24,B40,B15,B56,B67,B27,DR9,DR12,DR14,and DR16alleles seem to contribute to the genetic sus-ceptibility for children with ALL,especially B40allele,which is highly correlated while HLA-B48,DR7,DR15seems to contribute to its genetic resistance.