AM真菌与拮抗细菌的互作及对番茄根系酚类物质的影响

Interactions Between AM Fungus and Antagonistic Bacterium and Their Effect on Phenolic Content in Tomato Plant Root

在两室根箱系统中以番茄为供试植物,接种丛枝菌根(AM)真菌Glomusversiforme和/或拮抗细菌,研究两者在根际的互作及对根细胞壁结合态酚的影响,结果表明:拮抗细菌对菌根侵染率有轻微的促进作用,菌丝密度因接种拮抗细菌而增加,而接种AM真菌降低了拮抗细菌在根面、根际的定殖,前者由7 94×106g-1降低到3 75×106g-1,后者由8 08×105g-1降低到2 87×105g-1,其影响甚至延伸到根面以外2cm的范围;AM真菌和拮抗细菌都能提高根细胞壁结合态酚类物质的含量,但后者的增幅更大.可以认为,拮抗细菌对AM真菌有协同作用,双接种有可能促进AM真菌抗病功能的进一步发挥.

In a two-compartment rhizobox system, tomato plant(Lycopersicon esculentum Mill.) was inoculated with Glomus versiforme, an arbuscular mycorrhizal(AM) fungus, and/or an antagonistic bacterium to investigate their interactions in the rhizosphere and their effect on the cellwallbound phenolics of the host plant. Results indicated that the antagonistic bacterium improved the mycorrhizal infection rate slightly, and the hyphae length density was increased. Inoculation of AM fungus decreased the bacterial population on the root surface and in the rhizosphere, and the effect extended to 2 cm distance from the rhizoplane. Inoculation with both microbes increased the root cell wall-bound phenolics content, with a greater increase compared to bacterial inoculation. It is suggested that dual inoculation of AM fungus and antagonistic bacterium should enhance the pathogenresistance function of AM fungus.