无细胞短棒状杆菌纳米级制剂抗肿瘤作用及机理研究

Anti-Tumor Effects and Mechanism of the Product from Corynebacterium Parvum at Nano Scale (NCPP)

目的 :研究无细胞短棒状杆菌纳米级制剂 (NCPP)体内抗肿瘤作用及机理。方法 :小鼠腹腔注射NCPP与短棒状杆菌制剂 (CPP) 14d后测定脾指数。小鼠腹腔注射艾氏腹水癌细胞 ,于注射癌细胞前 1d及注射后次日起 ,连续 (间隔 1d)腹腔注射NCPP与CPP 5次 ,计算小鼠存活率。小鼠腹腔注射NCPP和CPP 10d后测定腹腔巨噬细胞 (Mφ)吞噬率、吞噬指数、过氧化氢 (H2 O2 )和一氧化氮 (NO)水平、脾脏自然杀伤 (NK)细胞杀伤率及T细胞增殖指数。结果 :NCPP组和CPP组与对照组相比 :均有明显的抑瘤作用 ;脾指数均大于 2 .0 ;腹腔Mφ吞噬率、吞噬指数、产生过氧化氢 (H2 O2 )和一氧化氮 (NO)水平均有显著性提高 (P <0 .0 1) ;脾脏NK细胞杀伤率均有明显提高 (P <0 .0 5 ) ;脾脏T细胞增殖指数均无明显差异。上述所有实验中 ,NCPP和CPP 2组间均无明显差异。结论 :NCPP具有与CPP相同的脾激活及抑瘤作用 ,其机理主要是通过激活Mφ及NK细胞吞噬、杀伤功能所致。

Objective: To investigate the in vivo anti-tumor effect and its mechanism of NCPP. Methods: Ehrlich′s ascites carcinoma cells were injected into the peritoneal cavity of mice. On the day before the injection and then, every other day, NCPP and Corynebacterum Parvum( CPP ) were given in the same way, total 5 times. The survival rates of the tumor-carrying mice were analyzed. 10 days after the injection of NCPP and CPP, data were collected, including the phagocytic index and its rate of the macrophages, the level of the hydrogen peroxide and nitric oxide produced by the macrophages, splenic natural killing (NK) cell′s activity, and the proliferation of T cells. And splenic index was evaluated on the 14th day after the injection of NCPP and CPP. Results: Compared with the control group, the survival rates of tumor-bearing mice were significantly increased in both NCPP and CPP groups; NCPP and CPP groups induced higher levels of splenic index (>2 in everage); higher levels of macrophage activity (P<0.01), and splenic NK cell activity (P<0 05), However, there was no difference in all the parameters mentioned as above between NCPP group and CPP group. Conclusions: This study shows that NCPP plays the same role as CPP in the tumor inhibition and splenic stimulation,mainly through the activiting macrophage and NK cells.