MCMV不同途径感染免疫缺损性小鼠体内效应的研究

Effects of Different Inoculations on Infection of MCMV in SCID Murine

通过血管注射、腹下注射、唾液腺注射等3种不同途径将野生型小鼠巨细胞病毒(murinecytomegalovirus,MCMV)感染免疫缺损型小鼠CM17SCID(severecombinedimmunodeficiency,严重免疫缺损综合症),在感染后不同的时间内分别取唾液腺、脾、肝、肺和肾脏测定其病毒滴度,以及测定感染后SCID小鼠的死亡率.同时通过唾液腺注射RvM43突变株,测定病毒在唾液腺中的滴度.结果显示:经尾部血管途径注射的体内唾液腺、肺、脾、肝和肾脏的病毒滴度高峰期和SCID小鼠死亡时间均显著性早于经腹下注射、唾液腺注射途径.除唾液腺器官外,经唾液腺注射途径肺、脾、肝和肾脏的病毒的出现时间晚于经尾部血管和腹下注射途径.经唾液腺注射后,唾液腺中突变型RvM43在各时间点的病毒滴度及高峰出现时间与野生型相同.由此可知,从唾液腺感染小鼠后MCMV病毒在唾液腺中的生长不受M43基因突变的影响,小鼠巨细胞病毒不同途径感染免疫缺损型小鼠CM17SCID的体内生物学效应有差异.因此有必要通过不同途径感染宿主来研究MCMV基因的体内功能.

The SCID mice were infected with MCMV by intraperitoneal,tail venous and salivary gland injection,respectively.The salivary glands,spleens,livers,lungs and kidneys were harvested and sonicated.Viral titers in these organs were determined by plaque assays.The mortalities of the mice were determined by different inoculations.The peaks of viral titers in salivary gland,spleen,liver,lung and kidney appeared at 14 days after inoculation by tail venous injection,and at 21 days after inoculation by intraperitoneal,or salivary gland injection.The virus inoculated by salivary gland injection were detected significantly later than that inoculated by intraperitoneal or tail venous injection in these organs except in salivary gland.All mice infected from tail venous died within 21 days after infection,whereas those infected by intraperitoneal and salivarygland injection died within 28 days after infection.RvM43 exhibited a titer similar to that of the wildtype virus in salivary gland by salivary gland injection.M43 appeared to be not essential for viral growth in vivo in salivary gland by salivary gland injection.The different inoculations with MCMV showed different infective effects on SCID murine host.