rhG—CSF基因工程菌发酵中pH值对表达率的影响

The Affection Of Medium's PH Value To The Expression Of The Aim Protein In rhG—CSF Fermentation

本文对重组人粒细胞集落刺激因子(rhG—CSF)基因工程菌的摇瓶发酵和发酵工艺进行了研究。结果表明,在摇瓶发酵中梯度调低培养基 pH 值可以提高产物的表达率;在发酵罐发酵工艺中适当调高培养基 pH 值可以提高产物的表达率。

This essay studies mainly the different optimum PH value in the two kinds of fer- mentation of Recombinant Human Granulocyte Colony Stimulating Factor's E.coli which include erlenmeyer fermentation and bioreactor fermentation.We conclude that reducing the pH value of medium can improve the expression of the aim protein in erlenmeyer fermentation,and raising the pH value of medium can improve the expression of the aim protein in bioreactor fermentation.