子宫内膜异位症患者腹腔液和在位子宫内膜血管内皮生长因子表达的研究

Expression of vascular endothelial growth factor in the peritoneal fluid and eutopic endometrium of patients with endometriosis

目的 :探讨血管内皮生长因子 (VEGF)在子宫内膜异位症 (EMT)发病中的意义。方法 :用酶联免疫吸附法测定 1 9例EMT患者和 2 1例正常妇女 (对照组 )腹腔液中VEGF水平。用相对定量RT PCR测定 1 5例EMT患者和 1 2例正常对照妇女在位子宫内膜VEGF的表达水平。结果 :EMT组腹腔液VEGF水平较正常对照组显著升高(P <0 .0 5 ) ,EMT组VEGF增殖期较分泌期升高 (P <0 .0 5 ) ,对照组无周期性变化 (P >0 .0 5 ) ;EMT组的在位子宫内膜VEGF的表达明显高于对照组 (P <0 .0 0 1 ) ,增殖期和分泌期两者差异无显著性 (P >0 .0 5 )。结论 :EMT患者腹腔液和在位子宫内膜VEGF升高 。

Objective To investigate the signficance of vascular endothelial growth factor (VEGF) on the etiology of endometriosis. Methods The level of VEGF in the peritoneal fluid from women with endometriosis ( n =19) and non endometriosis (n =21) was studied by ELISA. The expression of VEGF of eutopic endometrium collected from women with endometriosis ( n =15) and non endometriosis ( n =12) was determined by relative quantity RT PCR . Results The level of VEGF in the peritoneal fluid in the endometriosis was higher than that of the controls (P<0.05). Cyclic variation in VEGF concentration was seen in the peritoneal fluid from patients with endometriosis,and the VEGF concentration in the proliferative phase was significantly higher than that in the secretory phase (P< 0.05). No cyclic variation in VEGF was seen in the control group (P>0.05). The expression of VEGF in the eutopic endometrium with endometriosis was significantly higher than that of the controls (P< 0.001). There was no significant difference between the proliferative and secretory phase in endometriosis (P> 0.05) . Conclusion The increase of VEGF in the peritoneal fluid and eutopic endometrium with endometriosis may be important to the occurrence and development of endometriosis.