Fas-670基因多态性与炎症性肠病的相关性研究

Association of Fas Gene Promoter-670 Polymorphism with Chinese Patients with Inflammatory Bowel Disease

目的 :研究Fas基因 6 70位点多态性在中国汉族人群中的分布 ,探讨其与炎症性肠病的相关性。方法 :采用聚合酶链反应 限制性片段长度多态性方法 ,检测 5 0例炎症性肠病患者与 12 4例正常对照者Fas基因 6 70位点基因型及等位基因频率 ,分析该基因多态性与炎症性肠病之间的关系。结果 :炎症性肠病患者Fas基因型及等位基因频率与正常对照组比较无显著性差异。炎症性肠病组GG基因型频率与对照组相比有增高趋势 (2 8%vs 15 % ,P=0 .0 85 6 ) ,但差异无显著性。按性别分层后发现正常男性AA基因型频率显著高于正常女性 (4 0 %vs 19% ,P =0 .0 2 5 8)。溃疡性结肠炎组和克罗恩病组间Fas基因型和等位基因频率无显著性差异。而且Fas基因型与溃疡性结肠炎病变范围无关。结论 :Fas基因多态性与炎症性肠病的易感性无关。

Objective: To study distribution of Fas gene 670 polymorphism in Chinese Han population and the association with Chinese patients with inflammatory bowel disease (IBD). Methods: Fas 670 polymorphism was genotyped in 50 Chinese Han patients with IBD and 124 healthy controls by polymerase chain reaction and restriction fragment length polymorphism. Results: There were not overall significant differences in genotype and allele frequencies between the IBD and the controls. IBD patients displayed a slightly higher frequency of the GG homozygous genotype than healthy controls, but the difference was not significant (28% vs 15%,P=0.085 6)?The frequency of the AA genotype was significantly higher in male healthy controls than in female healthy controls (40% vs 19%,P=0.025 8). There were not significant differences in genotype and allele frequencies between the ulcerative colitis and Crohn's disease, as well as between left sided colitis and pancolitis in ulcerative colitis. Conclusion: Fas 670 polymorphism is not associated with Chinese Hubei Han patients with IBD. The AA genotype may have gender difference.