藏红花红O对家兔谷胱甘肽S-转移酶亚型活性的影响

Effects of Safranine O on the Activities of Glutathione S-Transferase Subtypes in Rabbits

目的 :观察藏红花红O对家兔血清、尿谷胱甘肽S 转移酶 (GST)及亚型α、μ、π活性的影响。方法 :家兔静脉注射藏红花红O建立肾损模型 ,采用紫外分光光度法连续动态测定血中GST及其亚型α、μ、π活性。同时与尿GST亚型活性及血尿素氮 (BUN)水平进行比较。结果 :血清、尿GST均于给药 6h后开始升高 ,12h升至最高。血GST π也于给药 6h后升高 ,9h升至最高 ,达正常对照的 3.5倍 (P <0 .0 5 )。尿GST π、尿GST α与其改变基本一致。血清及尿GST μ均无明显改变。血BUN于 15h开始升高 ,此后持续升高并维持在高水平上 ,且与GST改变无相关性。结论 :藏红花红O肾损家兔血清及尿GST及其不同亚型水平的改变 ,提示GST可作为早期诊断肾功能受损的指标 。

Objective: To investigate the effects of Safranine O on the activities of glutathione S transferase (GST) and its subtypes GST α,GST μ, GST π in the serum and urine of rabbits. Methods: The animal model of acute nephropathy was established by administered Safranine O (iv,30 mg) to rabbit. The fluctuation of GSTs activities within 15 h was measured by ultraviolet colormetry. At the same time,the levels of serum creatinine (Cr) and blood urea nitrogen (BUN) were determined. Results: After administered Safranine O 6 h,the GST activities of serum and urine both began to increase,and 12 h later,reached the climax. The serum GST π was also increased 6 h later,and the value of 9 h was exhibited to be a 3.5 fold of the 0 h value (P<0.05 ). The fluctuation of urine GST π,and GST α was similar to the serum GST π. The GST μ of serum and urine were unchanged. Blood urea nitrogen (BUN) was increased 15 h later,and maintained at a considerable higher level. However,the correlation between GST and BUN had no statistic significance. Conclusion: The measurement of GST activity could be used to diagnosis the renal insufficiency at early stage. Furthermore,it might provide some evidences about the position and extent of impaired kidney.