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吡那地尔对体外培养大鼠大脑皮层神经细胞缺氧缺糖损伤的保护作用 被引量：1

Protective Effects of Pinacidil on Glucose-Oxygen Deprived Injury in Cultured Cortical Neurons in Rat
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摘要 目的 :探讨ATP敏感性K+ 通道 (KATP)开放剂吡那地尔 (pinacidil,Pin)对缺氧缺糖再复氧损伤大鼠大脑皮层神经细胞的保护作用。方法 :体外培养大鼠大脑皮层神经细胞 ,细胞培养至 10d ,建立神经细胞缺氧缺糖损伤模型 ,观察Pin及KATP阻断剂格列苯脲对缺氧缺糖不同时间 ,再复氧 2 4h后细胞死亡率、丙二醛 (MDA)含量、超氧化物歧化酶 (SOD)活力的影响。结果 :缺氧缺糖、再复氧后大鼠的神经细胞死亡率均显著升高、MDA生成增多、SOD的活力下降 ,Pin干预后 ,细胞死亡率下降、MDA生成减少、SOD的活力升高 ;格列苯脲能拮抗Pin这种保护作用。结论 :Pin对缺氧缺糖损伤神经细胞具有保护作用 。 Objective: To investigate the protective effects of ATP sensitive potassium channel (K ATP ) opener Pinacidil on glucose oxygen deprived injury in cultured cortical neurons in newborn rats. Methods: When primary cultured neurons were exposed to glucose oxygen deprived condition for 4,8,16 h,reoxygen 24 h,the percentage of cell death and content of malonicaldehyde(MDA) and superoxide dimutase(SOD) were measured. Results: Pinacidil (10 -6 mol·L -1 ) reduced the percentage of cell death. It also prevented the elevation of MDA and increased the activities of SOD. K ATP agonist Glibenclamide could prevent the protective effects of Pinacidil. Conclusion: These results suggest that K ATP opener has neuronal protective effect,partly because it can suppress the generation of lipid peroxide and increase the activities of antioxidant enzymes.
作者 王华 麦根荣 陈宝芳 李涛 王卫民 潘同国 杨勇 谢集建
机构地区 武汉大学人民医院儿科 湖北省十堰市太和医院儿科 湖北省十堰市太和医院分子生物学实验室
出处 《武汉大学学报（医学版）》 CAS 2003年第3期258-261,T001,共5页 Medical Journal of Wuhan University
关键词 吡那地尔 体外培养 大鼠 大脑皮层 神经细胞缺氧缺糖损伤 保护作用 pinacidil glibenclamide ATP sensitive potassium channel cell culture
分类号 R722.12 [医药卫生—儿科]
  • 相关文献

参考文献8

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二级参考文献7

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共引文献2

同被引文献10

  • 1Dunn-Meynell AA, Rawson NE, Levin BE. Distribution and phenotype of neurons containing the ATP-sensitive K+channel in rat brain. Brain Res, 1998,814:41-54.
  • 2Yamada K, Ji JJ, Yuan H, et al. Protective role of ATP-sensitive potassium channels in hypoxia-induced generalized seizure.Science, 2001, 292 : 1543-1546.
  • 3Choi DW. Ionic dependence of glutamate neurotoxicity. J Neurosci, 1987,7: 369-379.
  • 4Sakai K, Yamagata T, Teragawa H, et al. Nicorandil enhances myocardial tolerance to ischemia without progressive collateral recruitment during coronary angioplasty. Circ J, 2002,66: 317-322.
  • 5Ye GL, Leung CK, Yung WH. Pre-synaptic effect of the ATP-sensitive potassium channel opener diazoxide or rat substantia nigra pars reticulata neurons. Brain Res, 1997,753:1-7.
  • 6Fujita H, Takizawa S, Nanri K, et al. Potassium channel opener reduces entracellular glutamate concentration in rat focal cerebral ischemia, Brain Res Bull, 1997,43: 365-368.
  • 7Copin JC, Reola LF, Chan TY, et al. Oxygen deprivation but not a combination of oxygen, gluose, and serum deprivation induces DNA degradation in mouse cortical neurons in vitro: Attenuation by transgenic overexpression of Cu Zn-superoxide dismutase. J Neuotrauma, 1996,13: 245-244.
  • 8Ishida H, Hirota Y, Genka C, et al. Opening of mitochondrial K(ATP) channels attenuates the ouabain-induced calcium overload in mitochondria. Circ Res,2001,89:856-858.
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  • 10姚裕家,李顺品,李炜如,陈娟,唐瑟,杨凡,王嵋景.缺氧缺血性脑病血和脑脊液LDH、CK及同工酶分析和相关性研究[J].中华围产医学杂志,1999,2(1):27-29. 被引量：37

引证文献1

武汉大学学报（医学版）
2003年 第3期

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