T淋巴细胞rDNA转录活性在恶性肿瘤中的表现及临床意义

Manifestation and clinical significance of rDNA transcription activity analysis of T lymphocytes in cancers

目的 :探讨外周血T淋巴细胞rDNA转录活性在恶性肿瘤中的表现及临床意义。方法 :以外周血T淋巴细胞核仁形成区嗜银蛋白 (AG NORs)作为T淋巴细胞rDNA转录活性分析指标 ,采用KL型肿瘤免疫图像分析系统对 1 0 0例正常健康者 ,2 63例肿瘤患者外周血T淋巴细胞检测的IS %值 (T淋巴细胞核仁银染面积与细胞核面积比值 )进行分析。结果 :外周血T淋巴细胞IS %值在正常人与肿瘤患者之间的表现差异有显著性 (P <0 .0 1 ) ;带瘤的和不带瘤的恶性肿瘤患者之间、健康者中不同年龄组之间差异有非常显著性 (P <0 .0 1 ) ;不同性别的健康者及恶性肿瘤患者中、不同部位肿瘤之间、不同细胞类型肿瘤之间、肿瘤组中不同年龄组之间差异无显著性 (P >0 0 5)。结论 :外周血T淋巴细胞IS %值与肿瘤细胞的存在与否有直接的关系 ,与性别、不同部位肿瘤、不同细胞类型无关 。

Purpose:To study the manifestation and clinical significance of rDNA transcription activity analysis of T lymphocytes in cancer patients.Methods:We analyzed the activity of the Ag NORs as one of the rDNA transcription activities in T cell of 100 normal human and 263 tumor patients by KL image system.Results:There were significant differences in the IS%of T lymphocytes in peripheral blood between normal human and cancer patients( P <0 01). The same results can be observed between the patients with tumor and without tumor residual as well as in different ages of normal human ( P <0 01).There were no significant differences in normal persons or cancer patients of different ages as well as in different position and the cell type of cancer ( P >0.05).Conclusions:There were significant differences in IS% of T lymphocytes in peripheral blood between normal human and cancer patients. But there was no relationship with the gender of the cancer patients, position and the cell type of tumor. So it can be one of the approaches to diagnose cancer and to monitor the progress. [