重庆地区骨质疏松症维生素D受体基因与骨密度关系的初探

The relationship between vitamin D receptor gene and bone mineral density in osteoporosis in Chongqing area

目的 探讨维生素D受体 (vitaiminDreceptor,VDR)基因多态性在重庆地区骨质疏松症人群中的分布 ,分析VDR基因型与骨密度的关系。方法 用聚合酶链反应限制性片段长度多态性 (polymerasechainreaction restrictionfragmentlengthpolymorphism ,PCR RFLP)技术检测了 15 9例绝经后妇女的维生素D受体基因多态性 ;采用单光子骨密度仪测定受试者非优势臂桡尺中下 1/ 3交界处桡骨骨密度 (BMD)。结果 骨质疏松组VDR基因型bb、Bb、BB分别为 83.2 5 %、16 .75 %、0 % ,非骨质疏松组为 84 .6 1%、15 .39 %及 0 % ,两组无统计学意义 (P >0 .0 5 ) ;骨质疏松组骨密度值 :bb基因型为 (0 .4 75± 0 .0 9)g/cm2 ,Bb基因型为 (0 .4 73± 0 .10 )g/cm2 。两种基因型的骨密度值无统计学差异。结论 就目前调查例数看 ,本地区骨质疏松患者骨密度与VDR基因型频率分布差异无显著性。

Objection To determine the frequency distribution of vitamin D receptor (VDR) genotype in osteoporosis in Chongqing area and to evaluate the correlation between VDR gene and polymorphisms and bone mineral density (BMD) .Methods Polymerase chain reaction(PCR) and restriction analysis were used to determine VDR genotypes in 159 postmenopausal women .their under one third of radioulnar in no dominant arm BMD was measured by single photon absorptimetry (SPA).Results The frequency distribution of the VDR genotype in osteoporosis was: bb ,Bb ,and BB genotype accounted for 83.25%, 16.75% , and 0% respectively; In no osteoporosis, they were 84.61% , 15.39% ,and 0% respectively ( P >0.05) .The BMD in osteoporosis of different genotypes were (0.475±0.09)g/cm 2 (bb),(0.473±0.10)g/cm 2 (Bb)respectively ( P >0.05). Conclusion There is no association between VDR genotype and BMD in osteoporosis in Chongqing area.