肺癌患者念珠菌感染快速检测方法的评价

EVALUATION OF RAPID IDENTIFICATION METHODS OF CANDIDA FOR THE LUNG CARCINOMA PATIENTS

目的评价念珠菌显色培养基 ,为测定肺癌患者念珠菌感染和选择用药提供快速准确的方法。方法采用显色培养基与沙保培养基同时对痰、气管分泌物进行分离鉴定 ,并通过常规生化实验和快速酵母反应板上机测定 ,将二种测定方法与显色培养基鉴定效率进行比较。结果显色培养基在 1 8h～ 30h内鉴定率为 77%、88%、1 0 0 % ,高于生化实验 (0、1 3.5 %、38.5 % )和快速酵母反应板 (1 0 .4 %、76 %、89.6 % ) ,对杂菌的抑制作用也优于沙保培养基。结论显色培养基测定念珠菌感染 ,操作简便 ,结果易测定 ,准确度高 。

ObjectiveTo supply a rapid and accurate method for identifying Candida infection and selecting the corresponding therapeutic drugs to lung carcinoma patents,the colorable candida culture medium was evaluated.MethodsPatient′s sputum and respiratory secretion were separated and identified simultaneously by the colorable culture medium and Sabourand culture medium.The identification efficiencies were compared among the culture medium test,the routine biochemical test and the rapid Yeast reaction panel test by the computer.ResultsWith in 18 h～30 h,the identification efficiency by colorable culture medium method was 77%,88% and 100% respectively,which was superior to the identification efficiency by biochemical method (0%, 13.5% and 38.5% ) and rapid Yeast reaction panel method ( 10.4% ,76% and 89.6% ).The non candida repression by the colorable culture medium was also superior to the Sabourand culture medium.ConclusionThese results suggest that it is a simple,convenient,sensitive and reliable method to identify the candida infection or the mixed Candida infection by the colorable culture medium test.