淡豆豉提取物的体外溶栓特性研究

Study on the fibrinolytic speciality in vivo of extract from Dochi

为了探讨各种因子对豆豉溶栓酶体外溶栓能力的影响 ,将豆豉溶栓酶放置于不同温度、酸碱度、金属离子强度及各种不同的化学物质中 ,测定其溶栓能力的大小。再加入各种类型的抑制剂 ,测定其溶栓能力的变化情况。以未经任何处理的同批次豆豉溶栓酶为对照组。结果豆豉提取物的最适存放温度为 4 5℃以下 ,最适pH为 8,Mn2 +、Ca2 +、Mg2 +显然对酶活有激活作用 ,添加明胶对溶栓酶活力起稳定作用。PCMB、TCLK、PMSF、胰酶抑制率几乎达 10 0 %。EDTA、β -巯基乙醇对此酶活性影响不大。

In order to study the factors affected on the fibrinolytic ability of Dochi fibrinolytic enzyme,to determine the fibrinolytic activity of Dochi fibrinolyric enzyme at various liquid environment such as different temperatures,pH,metal ions,various chemicals and inhibitors.The enzyme is treated as control.The extract from Dochi should be kept at the temperature below 45℃,the optimal pH value is 8,Mn 2+ ? Ca 2+ ?Mg 2+ can enhance the fibrinolytic activity,PCMB?TCLK?PMSF?trypsinase can inhibit the activity of fibrinolytic enzyme,EDTA and β-thiol ethy1 acid do not affect the fibrinolytic activity obviously.The study provides a reliable basis for the mass production of fibrinolytic enzyme.