猪血中IgG的分离、纯化放大试验工艺研究

介绍以放大的盐析法从猪血中提取IgG。通过正交试验,结合721分光光度计吸光率测定与电泳分析,确定最佳工艺条件为:pH7.0～7.3,温度40℃,盐析时间1h,搅拌转速40r/min。盐析沉淀采用PBS液错流管渗析脱NH4+及部分杂质,再作超滤设备选型、工艺流程设计、工艺条件正交试验,获浓缩液,冷冻干燥,测得IgG(纯度≥95%)收得率1.16%,为工业化生产推广探索工艺及操作参数。

In this article,the authors studied the scale -up technology of isolation and purification of IgG from pig blood.According to the orthogonal experiments,spectrophotometer and electrophorestic analysis,the optimal technological conditions are:pH7.2～7.3,temperature is40℃,time of salting out one hour,and speed of mill40r /min.The dialysis of salting out deposition was carried out by PBS crosscurrent flow to delete NH 4+ and some impurities.Then the lectotype of ultrafiltration equipment,desig n of the processing technolog y and technolog ical conditions were decided by orthog onal experiments.By freeze -drying,the yield of Ig G(purity≥95%)is1.16%.This study supplies the technolog y and parameters to industry.